首页> 外国专利> Method of in vitro cultivation to increase the biomass and increase the number of Seedlings of varieties and rootstocks of Prunus spp., Prunus avium.

Method of in vitro cultivation to increase the biomass and increase the number of Seedlings of varieties and rootstocks of Prunus spp., Prunus avium.

机译：一种通过体外培养增加生物量，增加李子，李子品种和砧木幼苗数量的方法。

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摘要

The present invention is an in vitro culture method to increase both the biomass and the number of seedlings in a number varieties and rootstock types of Prunus spp., particularly Prunus avium, and the use of said culture method, which is a useful means of micropropagating in vitro any variety and rootstock type of Prunus spp., in particular Prunus avium.

机译：本发明是一种体外培养方法，其用于增加李属（Prunus spp。）特别是鸟李（Prunus avium）的多个品种和砧木类型中的生物量和幼苗的数量，并且所述培养方法的用途是微繁殖的有用手段。在体外，李属的任何变种和砧木类型，特别是鸟李。

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公开/公告号CL2016002945A1

专利类型
公开/公告日2017-06-09

原文格式PDF
申请/专利权人 BIOTECNOLOGÍA FRUTÍCULA S.A. (25%);INSTITUTO DE INVESTIGACIONES AGROPECUARIAS INIA (75%);
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申请/专利号CL20160002945
发明设计人 TAPIA RODRIGUEZ EDUARDO ANDRES;GODOY GONZALEZ SEBASTIAN IGNACIO;PRIETO ENCALADA HUMBERTO GODOFREDO;
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申请日2016-11-18
分类号A01H4;
国家 CL
入库时间 2022-08-21 13:39:41

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1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

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