RECOMBINANT PROTEINS EXPRESSED IN ESCHERICHIA COLI AS ACTIVE INGREDIENTS IN VACCINES AGAINST INFLUENZA A H1N1 OUTBREAK 2009 AND PROCESS FOR PRODUCING THE SAME.

摘要

The present invention particularly refers to a recombinant protein which is specifically recognized by antibodies in the serum of patients infected with Influenza A H1N1 outbreak 2009 and which is proposed as a vaccine or as a main active ingredient of a vaccine produced in Escherichia Coli against Influenza A H1N1 that caused the epidemic outbreak 2009. Said protein is a fragment of the Hemagglutinin protein of the Influenza A H1N1 outbreak 2009 expressed in Escherichia coli strains. Additionally, the invention presents and protects a process through which said main active ingredient for a recombinant vaccine against Influenza A H1N1 causing the epidemic outbreak 2009 is obtained and purified through E coli cultures. The proteins including in the structure thereof the amino acid sequence SEC ID No. 1 or SEC ID No. 2 or SEC ID No. 3 are particularly protected. The use of said proteins as vaccines or active ingredients of a vaccine against Influenza A H1N1 outbreak 2009 is also protected. The invention also protects the process through which the aforesaid protein is produced, including a suitable level of biological activity and at amounts and levels of purity that ensure the proposed process to be applied in the industry.