INITIATORS AND PROBES FOR THE MOLECULAR AMPLIFICATION AND DETECTION OF ARN OF THE INFLUENZA AH1N1 VIRUS OUTBREAK 2009.

摘要

Described is a novel system for the molecular diagnosis of the influenza virus type A, and mainly of the influenza AH1N1 virus outbreak 2009 by RT/PCT (Reverse transcription polymerase chain reaction). The system is based on the specific recognition of a highly preserved zone in variants of the influenza A virus (sequence SEC ID No. 1) and the specific recognition of a sequence of 363 nucleotides (sequence SEC ID No. 2) exclusively present in the viral variant of the influenza AH1N1 that caused the epidemic outbreak in 2009. The method may be implemented in versions of end-point multiplex RT-PCR. Alternatively, chemical compounds may be used for the fluorescent dye of the amplified DNA, the aforementioned protocol may be implemented in a real-time thermal cycler. The invention also includes the use of specific fluorescence emitting probes (SEC ID No. 7 and 13, or the complementary threads thereof) which recognize regions of the sequence SEC ID No. 2 (or the complementary threads there of), the same allowing the PCR method to have an improved performance in its real time version.