Separation process oligonucleotide of interest from a mixture

摘要

A method of separating a target oligonucleotide from a mixture of target oligonucleotide and one or more impurities comprising: providing a system of liquid-liquid chromatography mobile phase biphasic -phase stationary liquid comprising a first mobile phase and a liquid stationary phase containing at least one exchange substance that binds with the possibility of separation target oligonucleotide; causing the first mobile phase liquid transport the target oligonucleotide in a flow in connection and in contact with the liquid stationary phase in the column apparatus liquid-liquid chromatography so that the target oligonucleotide remains attached to the exchange substance in the phase liquid stationary; then move the target oligonucleotide from the liquid stationary phase to a second liquid mobile phase flowing in relation and in contact with the stationary phase through the column by means of a substance of displacement capable of displacing the target oligonucleotide from step liquid stationary to the second mobile phase, characterized in that the exchange substance is a salt of a secondary, tertiary or quaternary organic amine with a counter anion, of general formula: R1R2R3R4N + Xen which through the secondary, tertiary and quaternary sequence, respectively, two, three or four of R1, R2, R3 and R4 groups are independently C1-20 alkyl or substituted alkyl as fluoro- or trifluoromethyl substituted alkyl or benzyl and the others are hydrogen, and X is a halide anion.