METHOD FOR THE CRYOPRESERVATION OF CELLS, ARTIFICIAL CELL CONSTRUCTS OR THREE-DIMENSIONAL COMPLEX TISSUES ASSEMBLIES

摘要

The present invention belongs to the field of cryopreservation of cells andtissue cultures,more specifically it refers to a method for the cryopreservation and long termstorage ofcells, cell constructs or three-dimensional complex tissues assemblies. Themethods arebased on the use of a collagen cell carrier having a specific composition aswell as to avery specific thickness and appropriate mechanical properties which aremaintained afterthawing. The use of such collagen cell carrier (CCC) provides a very suitablesupport forthe cryopreservation and providing cells and tissue assemblies already adheredto amechanically stable and biocompatible support. The invention also refers tothe frozencollagen carrier-cells assembly and to the frozen artificial cell constructsor three-dimensional complex tissue assemblies obtainable by the method of theinvention and tothe use thereof after thawing.