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PSEUDO INTERNAL STANDARD METHOD, DEVICE AND APPLICATION FOR MASS SPECTROMETRY QUANTITATIVE ANALYSIS
PSEUDO INTERNAL STANDARD METHOD, DEVICE AND APPLICATION FOR MASS SPECTROMETRY QUANTITATIVE ANALYSIS
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机译:伪谱内标方法的定量分析方法,装置及应用
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摘要
A simulated internal standard method, device and application for mass spectrometry quantitative analysis. The method comprises steps: taking a standard, and formulating same into standard solutions with different concentrations by using a blank substrate of a sample to be tested, and then taking the same standard, and formulating same into internal standard solutions with one to several concentrations by using the blank substrate of the sample to be tested; processing the standard solutions and the internal standard solutions with different concentrations, through a sample processing step, into a standard sample and an internal standard sample; successively injecting the standard sample and the internal standard sample into a chromatography-mass spectrometry system to be analysed and measured; plotting a ratio of peak areas of the standard sample and the internal standard sample against the concentrations to obtain a calibration curve of simulated internal standard method quantification; and injecting the sample to be tested after being processed by the sample processing step into the chromatography-mass spectrometry system to be analysed and measured, and then comparing the peak area of a tested signal with the calibration curve to determine the concentration of the sample to be tested.
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