Pseudo internal standard methods, devices, and applications for mass spectrometric quantitative analysis

摘要

The present invention discloses a pseudo-internal standard method, device, and application for mass spectroscopic quantitative analysis. The method takes a standard by using a blank substrate for the sample to be tested, formulates it into a standard solution having a different concentration, and then uses the same standard by using a blank substrate for the sample to be tested. Taking an object and formulating it into an internal standard solution having one to several concentrations; processing a standard solution and an internal standard solution having different concentrations to a standard sample and an internal standard sample by a sample processing step; Standard and internal standard samples are injected into the chromatograph-mass spectrometry system for analysis and measurement, and a standard internal peak area and internal to establish a calibration curve for quasi-internal standard method quantification. Plotting the ratio of the standard sample peak area against the standard sample concentration, processing the unknown sample through the same sample processing stage, then analyzing and The sample detected by following the same steps as the standard sample analysis and the internal standard are injected into the chromatograph-mass spectrometry system, and the peak area ratio of the sample to the internal standard is calibrated to determine the concentration of the unknown sample. Comparing with the curve. [Selection] Figure 1