WHOLE-BODY TISSUE STABILIZATION AND SELECTIVE EXTRACTIONS VIA TISSUE-HYDROGEL HYBRIDS FOR HIGH RESOLUTION INTACT CIRCUIT MAPPING AND PHENOTYPING

摘要

In various embodiments, the present application teaches methods for tissue clearing in which tissues are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches ePACT, which is a protocol for enhanced tissue clearing via expansion. In some embodiments, the present application teaches visualizing a tissue that has been expanded via ePACT.