METHOD OF ACQUIRING TISSUE OPTICAL PARAMETERS OVER WIDE FIELD AND PERFORMING LIGHT REFLECTANCE IMAGING OF MICROSTRUCTURE

摘要

A method of acquiring tissue optical parameters over a wide field and performing light reflectance imaging of a microstructure. The method comprises a method of calculating reflectance of a turbid medium applicable at any spatial separation and in the entire spatial frequency domain, and a method of obtaining an optical parameter of the medium by measuring reflectance of the turbid medium at high and low spatial frequencies and then inverting the obtained optical reflectance measurements, wherein the inversion method can be a look-up table method or a formula-fitting method. The measurement of sub-diffusive and diffuse reflectance of turbid media can be used to determine the optical properties of a turbid medium and microstructures such as phase function over a wide field.