METHOD FOR SYNTHESIS OF PHARMACEUTICAL RECOMBINANT PROTEIN BASED ON INTEIN

摘要

Disclosed is a method for the synthesis of a pharmaceutical recombinant protein based on an intein, comprising the following steps: construction of vector DNA fragments expressing a polypeptide having a targeting function and a polypeptide having a toxic function in an immunotoxin, the vector DNA fragments of the two polypeptides respectively being linked to the N-terminus or C-terminus of the intein to form a fusion expression vector; taking the fusion expression vector described in step 1, and expressing same to obtain a fusion polypeptide A that fuses the N-terminus of the intein and a fusion polypeptide B that fuses the C-terminus of the intein; taking the fusion polypeptide A and the fusion polypeptide B, mixing them, and inducing the mixture under trans-splicing conditions corresponding to the intein to obtain the immunotoxin. The method only requires the preparation of different targeting part polypeptides and toxic part polypeptides, wherein same can be combined and then produce an immunotoxin that can target different targets and have different toxic mechanisms, with significant diversity and flexibility.