Screening method based on cell-free protein synthesis to determine the factors affecting the expression efficiency or activity of recombinant proteins

摘要

The present invention relates to a screening method for a protein expression or activity enhancing cofactor using cell-free protein synthesis. Specifically, in the production of a recombinant protein, the expression of a protein and the formation of a functional three-dimensional structure are affected by various factors. , relates to a screening method that greatly improves the productivity of a target protein having activity by sequentially expressing a cofactor protein and a target protein that help in the synthesis and functional structure of a specific protein.