Method for detection of Cochlodinium polykrikoides by sandwich hybridization integrated with nuclease protection assay and kit therefor

摘要

The present invention relates to a method and a kit for detecting a cochlear polyclicoids using nuclease protection assay integrated sandwich hybridization. More specifically, the present invention relates to a nuclease protection assay probe, a capture probe, And a method for detecting Cochlonidium polycliquiides contained in a sample by performing a sandwich hybridization integrated with a nuclease protection assay (NPA-SH) using a signal probe, Kit.According to the present invention, it is possible to detect Cochrlidium polyclicoids so as to be very accurately distinguished from other microalgae from a sample, and quantitative detection is also possible. Therefore, it is expected that the occurrence of C. polycliquiides can be predicted and prepared, and it is expected to contribute not only to the maintenance of stable marine ecosystem, but also to reduce unexpected damage of fisheries and aquaculture.;