Probe set for improving accuracy of detectection to target nucleic acid and the method for detecting target nucleic acid using the same

摘要

The present invention relates to a probe set for increasing detection accuracy for a target nucleic acid, a method for designing the probe set, and a method for detecting a target nucleic acid through melting curve analysis using the probe set .The probe set provided in the present invention enables the presence or absence of a target nucleic acid to be detected with very high accuracy. In addition, the probe set provided in the present invention can use fluorescent materials of various wavelength ranges as reporters, so that target nucleic acids at various sites in one nucleic acid sequence can be detected simultaneously, and target nucleic acids in various nucleic acid sequences can be detected Can be detected at the same time.;