Method for Rapid Bottle Cultivation Grifola Frondosa

摘要

The present invention relates to a method for cultivating a mushroom of a leaf mushroom, and more particularly, to a method for cultivating a mushroom of a leaf mushroom by shortening the production period of the mushroom by 10 days or more by establishing an optimized bottle cultivation condition based on the growth characteristics of the mushroom This is about bottle cultivation technology.According to the present invention, there is provided a method for cultivating a mushroom cultivated by a method comprising the steps of: preparing a culture medium by mixing 95 to 99% by weight of oak sawdust with 1 to 5% by weight of wheat bran; A second step of filling the medium prepared in the first step with 0.75 to 0.8 g / 1 cc into a bottle of 850 50 cc having an inlet size of 6.2 0.5 cm; The third step of primary sterilization of the cultivation bottle filled with the medium at 70 10 minutes at 105 5C and then the secondary sterilization at 120 10C for 110 10 minutes; A fourth step of cooling so that the deep portion temperature of the cultivating bottle becomes 10C or lower; A fifth step of inoculating 1 to 5 g of the mushroom seedlings into a cultivating bottle; The culture was maintained at 25 to 30C in the initial 10 2 days, at 23 to 25C in the middle stage 10 2 days, at 20 to 22C in the latter 10 2 days, Separating and culturing the cells by light irradiation; The temperature was maintained at 19 2C for the first 2 1 days and 17 2C for the next 13 3 days after removing the bottle cap. The humidity was 90 5% at the initial 4 1 days, And a seventh step of growing the strain at 80 5% for 5 2 days and 60 5% for 6 2 days at the latter stage.;