首页> 外国专利> IV A microorganism having enhanced levan fructotransferase productivity and a method of producing difructose anhydride IV using the microorganism

IV A microorganism having enhanced levan fructotransferase productivity and a method of producing difructose anhydride IV using the microorganism

机译:IV具有提高左旋果糖转移酶生产率的微生物和使用该微生物生产果糖酐IV的方法

摘要

The present invention relates to a nucleic acid-containing Levan fructitin-transferase-secretion expression cassette encoding a levan fructotransferase-encoding nucleic acid and a translational fusion partner (TFP), a vector containing the cassette, A recombinant microorganism containing the recombinant microorganism, and a process for producing a levansucrose transferase and a difructose anhydride IV using the recombinant microorganism.The recombinant microorganism of the present invention can secrete and express the recombinant levan fructosyltransferase at a good efficiency by using a protein secretion fusion factor and can be obtained by reacting the levan fructosyltransferase produced from the microorganism with levan, Can be fermented in a medium containing Levan to effectively produce Diprutosteranhydride IV.
机译:编码左果糖基转移酶的核酸和翻译融合伴侣(TFP)的含核酸的左果糖蛋白转移酶分泌表达盒,包含该盒的载体,包含重组微生物的重组微生物和本发明的重组微生物通过使用蛋白质分泌融合因子可以高效地分泌和表达重组左旋果糖基转移酶,并且可以通过使蛋白质与蛋白质反应而获得。由微生物与莱万产生的莱万果糖基转移酶,可以在含有莱万的培养基中发酵,以有效地生产双戊二酸酐IV。

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