METHOD FOR GENETIC POLYMORPHISM RAPID ANALYSIS FOR DETECTION OF GENETIC PREDISPOSITION TO BREAST CANCER

摘要

FIELD: biotechnology.;SUBSTANCE: method is for rapid analysis of genetic polymorphism to detect genetic predisposition to breast cancer (BC). 40 bases long oligonucleotides containing the genetic marker and not containing the genetic marker shown in Table 1, are immobilized on the microchip, 3 repetitions of each oligonucleotide, together with those shown in Table 3 from human housekeeping gene as a positive control and from genes of other organisms that have no homology in the human genome, as a negative control. Nucleic acids are selected from the blood sample, using the total RNA or DNA. A labeled probe is obtained for hybridization by reverse transcription reaction with simultaneous fluorescent labeling for RNA or nick translation labeling of DNA. The obtained probe is hybridized with oligonucleotides immobilized on the microchip. The non-specifically bound probe is washed under conditions discriminating against mutant genotypes. The microchip is scanned and the results evaluated.;EFFECT: increased specificity and reliability of the analysis and improved breast cancer risk assessment reliability.;3 tbl, 1 dwg