RECOMBINANT DNA FRAGMENT, ENCODING HUMAN ALPHA-FETOPROTEIN (AFP), CONTAINING 'SLOW' LEUCINE-ENCODING CODONS, EXPRESSION PLASMID, CONTAINING SAID FRAGMENT, SACCHAROMYCES CEREVISIAE CELL, TRANSFORMED WITH SAID PLASMID, AND METHOD FOR OBTAINING HUMAN AFP

摘要

FIELD: medicine.;SUBSTANCE: invention relates to field of biotechnology, can be used for obtaining human alpha-fetoprotein (AFP) with application of strains of Saccharomyces cerevisiae yeasts, containing recombinant AFP gene. Obtained are: recombinant human AFT, containing so-called "slow" codons, encoding leucine in conservative AFP sections, recombinant plasmid and Saccharomyces cerevisiae cell for expression and secretion of said recombinant AFP respectively.;EFFECT: invention makes it possible to increase production of active AFP.;9 cl, 16 dwg, 3 tbl, 6 ex