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METHOD FOR DIAGNOSIS OF DISEASE ACCOMPANIED BY INCREASED CELL DEATH, AND SET FOR ITS IMPLEMENTATION

机译:诊断伴随细胞死亡增加的疾病的方法及其实施方法

摘要

FIELD: medicine.;SUBSTANCE: to perform the method for diagnosis of diseases accompanied by increased cell death, a blood plasma sample is obtained, followed by isothermal amplification of blood plasma nucleic acids. Next, the nucleic acids are purified from the reaction mixture and the quantitative analysis of the nucleic acids by the polymerase chain reaction method is carried out in real time. The coefficient K is determined in the samples. Next, the coefficient K is compared with the reference value and the disease, accompanied by an increased loss of cells, is diagnosed at K value less than the reference value.;EFFECT: invention allows minimally invasive diagnosis of diseases accompanied by increased cell death, with high sensitivity, at any stage of the disease, including in the early stages, when clinical symptoms are absent.;52 cl, 5 tbl, 1 ex
机译:领域:药物:物质:执行用于诊断伴随细胞死亡增加的疾病的方法,获得血浆样品,然后等温扩增血浆核酸。接下来,从反应混合物中纯化核酸,并通过聚合酶链式反应方法对核酸进行实时定量分析。在样本中确定系数K。接下来,将系数K与参考值进行比较,并以小于参考值的K值诊断出伴随细胞丢失增加的疾病。效果:本发明允许对伴随细胞死亡增加的疾病进行微创诊断,在疾病的任何阶段(包括在没有临床症状的早期阶段)都具有高敏感性; 52 cl,5 tbl,1 ex

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