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Method of identification of the animal species mammal of origin of the meat or gelatin of a sample to be tested

机译:鉴定待测样品的肉或明胶来源的哺乳动物的动物的方法

摘要

The present invention relates to a method for identifying the bovine or porcine animal species of origin for meat or gelatin, possibly contained in a specimen of a foodstuff to be tested, wherein the following steps are carried out: a/ preparing a peptide preparation of a protein extract of whole proteins or a peptide extract from said specimen; b/ producing a spectrum of said preparation by mass spectrometry of the MALDI TOF type; c/ comparing the spectrum obtained in step b/ with at least one reference spectrum for a said extract of a meat reference specimen chosen at least from among bovine meat and porcine meat, or a gelatin reference specimen chosen at least from among bovine gelatin or porcine gelatin; and d/ identifying the presence of meat or gelatin, respectively, of the mammal animal species of origin for said tested sample as being that of a said reference animal species if, in the spectrum obtained in step b/ above, there are shared peaks comprising at least specific peaks of a said reference spectrum from step c/ above if applicable and/or by determining the proximity of the spectrum of the tested sample to a reference spectrum, preferably by hierarchical classification.
机译:本发明涉及一种鉴定可能包含在待测食品样品中的肉或明胶来源的牛或猪动物物种的方法,其中进行以下步骤:a /制备肽的肽制剂从所述标本中提取全蛋白质的蛋白质提取物或肽提取物; b /通过质谱法MALDI TOF产生所述制剂的光谱; c /将在步骤b /中获得的光谱与至少从牛和猪肉中选择的肉参考样品的所述提取物或至少从牛明胶或猪中选择的明胶参考样品的所述至少一种参考光谱进行比较明胶;如果在上述步骤b /获得的光谱中存在共有的峰,则d和d /分别将所述测试样品的起源的哺乳动物动物的肉或明胶的存在鉴定为所述参考动物的肉或明胶的存在。如果适用,和/或通过确定被测样品的光谱与参考光谱的接近度,最好通过分级分类,确定来自上述步骤c /的所述参考光谱的至少特定峰。

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