The present invention relates to a kit for amplifying and detecting a Chikungunya virus (CHIKV) nucleic acid sequence, comprising: (a) a first primer up to 100 bases long and comprising a target-complementary 3' terminal sequence consisting of 15-43 contiguous bases of SEQ ID NO:35, which is in the gene encoding the E1 protein, (b) a second primer up to 100 bases long and comprising a target-complementary 3' terminal sequence consisting of 15-41 contiguous bases of SEQ ID NO:56,which is in the polyprotein gene region, the target-complementary 3' terminal sequence of the first and second primers being fully contained within the sequences of SEQ ID NO:35 and SEQ ID NO:56, respectively, and the first and second primers optionally comprising a second primer 5' sequence that is not complementary to CHIKV nucleic acids; and (c) a hybridization probe for detecting a nucleic acid amplification product synthesized using the primers, wherein the primers and the hybridization probe are in packaged combination with each other.
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机译:本发明涉及一种用于扩增和检测基孔肯雅病毒(CHIKV)核酸序列的试剂盒,其包含:(a)长达100个碱基的第一引物,并包含由15-43个连续的靶互补的3'末端序列SEQ ID NO:35的碱基,位于编码E1蛋白的基因中;(b)第二个引物,长度不超过100个碱基,并包含由SEQ ID NO的15-41个连续碱基组成的靶标互补3'末端序列:56,其在多蛋白基因区域中,第一和第二引物的靶互补3'末端序列分别完全包含在SEQ ID NO:35和SEQ ID NO:56的序列中,并且第一和第二引物第二引物,其任选地包含与CHIKV核酸不互补的第二引物5'序列; (c)用于检测使用所述引物合成的核酸扩增产物的杂交探针,其中,所述引物和所述杂交探针彼此组合包装。
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