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STOICHIOMETRIC TUNING OF NUCLEIC ACID HYBRIDIZATION PROBES BY AUXILIARY OLIGONUCLEOTIDE SPECIES
STOICHIOMETRIC TUNING OF NUCLEIC ACID HYBRIDIZATION PROBES BY AUXILIARY OLIGONUCLEOTIDE SPECIES
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机译:辅助寡核苷酸物种的核酸杂交问题的化学计量学调节。
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摘要
This invention describes a method of controlling the hybridization yield of nucleic acid probes by adjusting the relative concentrations of auxiliary oligonucleotides to the probes and the targets. The auxiliary oligonucleotide is partially or fully complementary to either the probe or the target, and is released upon hybridization of the probe to the target.
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