ENGINEERING AND OPTIMIZATION OF IMPROVED SYSTEMS, METHODS AND ENZYME COMPOSITIONS FOR SEQUENCE MANIPULATION

摘要

PROBLEM TO BE SOLVED: To address a pressing need for alternative and robust systems and techniques for sequence targeting with a wide array of applications and provide related advantages.SOLUTION: The invention provides engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are compositions and methods related to components of a CRISPR complex particularly comprising a Cas ortholog enzyme.SELECTED DRAWING: Figure 1