METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELL FROM SOMATIC CELL, AND MEDIUM FOR CULTURING INDUCED PLURIPOTENT STEM CELL IN UNDIFFERENTIATED STATE

摘要

PROBLEM TO BE SOLVED: To provide a method for producing an induced pluripotent stem cell from a somatic cell, conveniently and inexpensively, without using a feeder cell.;SOLUTION: A method for producing an induced pluripotent stem cell from a somatic cell has a culture step in which: a nucleus initialization factor is introduced into a somatic cell, and cultured in a low oxygen environment without using a feeder cell. In the culture step, used is a medium containing at least one inorganic salt, at least one amino acid, at least one vitamin, D-glucose, sodium phenol red, sodium pyruvate, antibiotic, and serum.;SELECTED DRAWING: None;COPYRIGHT: (C)2018,JPO&INPIT