NUCLEIC ACID APTAMER WITH FokI NUCLEASE DOMAIN AFFINITY

摘要

PROBLEM TO BE SOLVED: To develop a method of more efficiently introducing an artificial nuclease with little off-target effect for genome editing.;SOLUTION: The method of introducing an artificial nuclease into a cell comprises: a step of binding the artificial nuclease containing a FokI nuclease as a DNA cleavage domain to the surface of a nanoneedle via a nucleic acid aptamer which specifically binds to the FokI nuclease domain; and a step of puncturing the cell with the nanoneedle bound to the artificial nuclease.;SELECTED DRAWING: None;COPYRIGHT: (C)2018,JPO&INPIT