Genetically modified immune effector cells and genetically engineered cells for expansion of immune effector cells

摘要

Chimeric antigen receptor (CAR) polypeptides (and immune effector cells that express CAR) comprising a modified hinge domain sequence are provided. Also provided are genetically engineered antigen-presenting cells (APCs) that express a transgene encoding a target antigen and human leukocyte antigen (HLA). In a further aspect, immune effector cells are provided that are selected for elevated mitochondrial reserve respiratory capacity. A chimeric antigen receptor (CAR) polypeptide comprising an antigen binding domain; a hinge domain; a transmembrane domain and one or more intracellular signaling domain (s), wherein the hinge domain comprises an IgG4-Fc sequence The chimeric antigen receptor (CAR) polypeptide, wherein the IgG4-Fc sequence comprises at least one mutation that reduces Fc receptor binding relative to wild-type IgG4-Fc. [Selection figure] None