Method for detecting multiple types of short-chain nucleic acids in a sample, combination analysis kit, and analysis kit supply management method

摘要

The detection method of the embodiment is a method for detecting a plurality of types of target nucleic acids in a sample. Each of the plurality of target nucleic acids is a short-chain nucleic acid containing a different target sequence. The method comprises: (a) preparing a long-chained nucleic acid set group for obtaining a long-chain nucleic acid for each target nucleic acid, a primer set for amplifying a plurality of long-chain nucleic acids in common, and a probe-immobilizing substrate; (b) Obtaining a group of long-chain nucleic acids comprising the target sequence or its complementary sequence, the corresponding first sub-long chain sequence, and the corresponding second sub-long chain sequence, (c) the long sequence Maintaining the nucleic acid group and the primer set under amplification conditions to obtain an amplification product group; (d) presence / absence and / or amount of hybridization between the amplification product group and the first probe. Detecting (e) detecting the plurality of target nucleic acids based on the results.