New means for enabling stable mass production of knockout individuals in large animals is also disclosed. The method of the present invention is a method for producing a nonhuman large mammal or fish (nonhuman animal) which produces a gamete derived from a different individual, wherein the function of the nanos 3 gene derived from the first non-human animal is inhibited Transplanting at least one pluripotent cell derived from a second non-human animal into a cleavage stage embryo having a genome that has been produced, preparing a chimeric embryo, generating the chimeric embryo to obtain an individual including. When a pluripotent cell having a genome in which a desired gene is knocked out is used as a pluripotent cell derived from a second non-human animal, reproductive reproduction in which a desired gene is knocked out in the first non-human animal It is possible to mass-produce knockout nonhuman animals easily by mating of such non-human animals.
展开▼
