DECOY NUCLEIC ACID CATIONIC LIPOSOME CARRIER AND PREPARATION METHOD THEREOF
展开▼
机译:诱饵核酸阳离子脂质体载体及其制备方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A preparation method of a Decoy nucleic acid cationic liposome carrier including: (1) mixing dioleoyl phosphoethanolamine and (2,3-dioleoyl-propyl)-trimethylamine in a mass ratio of 4:1 to 1:4, and adding an organic solvent to obtain a mixed solution through dissolution; (2) completely evaporating the organic solvent in the mixed solution obtained in step (1), dissolving the remaining solid fraction by using an HEPES buffer solution, firstly hydrating the solution for 30 to 60 min, and then ultrasonically processing the solution for 30 to 60 min; (3) filtering the mixed system obtained after processing in the step (2) by a membrane of 0.4 to 0.8 μm, then filtering the mixed system by a membrane of 0.03 to 0.2 μm, and preparing uniformly to distributed blank liposome with small grain size; and (4) mixing the blank liposome with protamine and Decoy nucleic acid according to a mass ratio of (50 to 120):(10 to 20):1, and incubating for 12 to 24 h at the temperature of 2° C. to 8° C. to form the complete Decoy nucleic acid cationic liposome carrier.
展开▼