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Fungus-Sourced High-Temperature Acid B-Glucosidase as well as Coding Gene and Application Thereof

机译:真菌来源的高温酸性B-葡萄糖苷酶及其编码基因及其应用

摘要

Provided are a fungus-sourced high-temperature acid β-glucosidase as well as a coding gene, and an application thereof. The provide β-glucosidase has the optimal pH value of 4.5 and the optimal temperature of 75° C., and maintains over 90% enzyme activity in the optimal condition after being processed at 60° C. for 1 h. The re-engineering yeast strain GS115/bgl3A of the coding gene comprising the β-glucosidase has high fermentation level.
机译:本发明提供了一种真菌来源的高温酸性β-葡萄糖苷酶及其编码基因及其应用。所提供的β-葡糖苷酶具有4.5的最佳pH值和75℃的最佳温度,并且在60℃下处理1h后在最佳条件下维持超过90%的酶活性。包含β-葡糖苷酶的编码基因的重组酵母菌株GS115 / bgl3A具有高发酵水平。

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