BROAD-SPECTRUM SEROLOGICAL DIAGNOSTICS AND USES THEREOF

摘要

In some embodiments, the present disclosure pertains to method of screening a biological sample for a plurality of diseases. In some embodiments, such a method comprises obtaining a biological sample from a subject in need thereof. In some embodiments, the biological sample comprises a plurality of biomarkers. In some embodiments, each of the plurality of biomarkers is specific for at least one disease. In some embodiments, the method comprises contacting the biological sample with a display library of peptides. In some embodiments, each peptide in the library may have a unique amino acid sequence. In some embodiments, each of the peptides is physically linked to a nucleic acid sequence that identifies of encodes the peptide. In some embodiments, at least one of the peptides is capable of binding to at least one of the biomarkers in the biological sample. In some embodiments, the method comprises separating the bound peptide particles from the unbound peptide particle. In some embodiments, the method comprises eluting the bound peptide particles from the bound state. In some embodiments, the method comprises determining the identity of the nucleic acid sequences physically linked to the bound peptide particles. In some embodiments, the method comprises comparing the sequences thus obtained to a database of sequences representing a plurality of biomarkers of diseases.