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Composition comprising cryopreservation medium and stem cells obtained by slow-freezing
Composition comprising cryopreservation medium and stem cells obtained by slow-freezing
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机译:包含冷冻保存培养基和通过缓慢冷冻获得的干细胞的组合物
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摘要
Cryopreserved stem cells with high cell viability after thawing were obtained by slow freezing using a DMSO-free cryopreservation medium. Stem cells and/or progenitor cells thereof are contacted with a DMSO-free cryopreservation medium, comprising between 4 v/v % and 25 v/v % of propylene glycol and between 1.0 w % and 10 w % of a sugar; and are subsequently subject to a slow-freezing process. Optionally, the cryopreservation medium may comprise serum albumin and/or hyaluronic acid.
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机译:通过使用不含DMSO的冷冻保存介质缓慢冷冻,可获得融化后具有高细胞活力的冷冻保存的干细胞。干细胞和/或其祖细胞与不含DMSO的冷冻保存液接触,该保存液包含4 v / v%至25 v / v%的丙二醇和1.0 w%至10 w%的糖;并随后进行缓慢的冷冻过程。任选地,冷冻保存介质可以包含血清白蛋白和/或透明质酸。
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