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MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium

机译:雪旺细胞条件培养液分化为神经元样细胞的骨髓间充质干细胞微小RNA变化

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摘要

Bone marrow-derived mesenchymal stem cells differentiate into neurons under the induction of Schwann cells.However,key microRNAs and related pathways for differentiation remain unclear.This study screened and identified differentially expressed microRNAs in bone marrow-derived mesenchymal stem cells induced by Schwann cell-conditioned medium,and explored targets and related pathways involved in their differentiation into neuronal-like cells.Primary bone marrow-derived mesenchymal stem cells were isolated from femoral and tibial bones,while primary Schwann cells were isolated from bilateral saphenous nerves.Bone marrow-derived mesenchymal stem cells were cul tured in unconditioned (control group) and Schwann cell-conditioned medium (bone marrow-derived mesenchymal stem cell + Schwann cell group).Neuronal differentiation of bone marrow-derived mesenchymal stem cells induced by Schwann cell-conditioned medium was observed by time-lapse imaging.Upon induction,the morphology of bone marrow-derived mesenchymal stem cells changed into a neural shape with neurites.Results of quantitative reverse transcription-polymerase chain reaction revealed that nestin mRNA expression was upregulated from 1 to 3 days and downregulated from 3 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group.Compared with the control group,microtubule-associated protein 2 mRNA expression gradually increased from 1 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group.After 7 days of induction,microRNA analysis identified 83 significantly differentially expressed microRNAs between the two groups.Gene Ontology analysis indicated enrichment of microRNA target genes for neuronal projection development,regulation of axonogenesis,and positive regulation of cell proliferation.Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrated that Hippo,Wnt,transforming growth factor-beta,and Hedgehog signaling pathways were potentially associated with neural differentiation of bone marrow-derived mesenchymal stem cells.This study,which carried out successful microRNA analysis of neuronal-like cells differentiated from bone marrow-derived mesenchymal stem cells by Schwann cell induction,revealed key microRNAs and pathways involved in neural differentiation of bone marrow-derived mesenchymal stem cells.All protocols were approved by the Animal Ethics Committee of Institute of Radiation Medicine,Chinese Academy of Medical Sciences on March 12,2017 (approval number:DWLI-20170311).
机译:在Schwann细胞的诱导下,骨髓源间充质干细胞分化为神经元。但是,关键的microRNA及其分化途径仍不清楚。本研究筛选并鉴定了Schwann细胞诱导的骨髓源间充质干细胞中差异表达的microRNA。条件培养基,并探索了涉及其分化为神经元样细胞的靶标和相关途径。从股骨和胫骨中分离出原代骨髓间充质干细胞,从双侧隐神经中分离出原代Schwann细胞。间充质干细胞在无条件的培养(对照组)和雪旺氏细胞培养液(骨髓源性间充质干细胞+雪旺氏细胞组)中培养。由雪旺氏细胞培养液诱导的骨髓间充质干细胞的神经分化通过延时成像观察。诱导后,骨髓的形态衍生的间充质干细胞变成具有神经突的神经形状。定量逆转录-聚合酶链反应的结果显示,在源自骨髓的间充质干细胞中,巢蛋白mRNA表达在1至3天上调,并在3至7天下调。雪旺细胞组。与对照组相比,骨髓间充质干细胞+雪旺细胞组中微管相关蛋白2 mRNA的表达从1到7天逐渐增加。诱导7天后,microRNA分析发现83个显着差异在两组之间表达microRNA。基因本体分析表明,microRNA靶基因富集,可用于神经元投射发育,轴突发生调节和细胞增殖的正向调节。《基因百科全书》和基因组通路分析表明,Hippo,Wnt,转化生长因子- β和Hedgehog信号通路可能与ne相关该研究成功地通过施旺细胞诱导技术从骨髓间充质干细胞分化而来的神经元样细胞的microRNA分析,揭示关键的microRNA和参与骨神经分化的途径进行了成功的microRNA分析。骨髓来源的间充质干细胞.2017年3月12日,所有方案均获中国医学科学院放射医学研究所动物伦理委员会批准(批准文号:DWLI-20170311)。

著录项

  • 来源
    《中国神经再生研究(英文版)》 |2019年第8期|1462-1469|共8页
  • 作者单位

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

    Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, China;

    Department of Orthopedics, Henan Provincial People's Hospital, Zhengzhou, Henan Province, China;

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

    Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 药物疗法、化学疗法;病理过程;
  • 关键词

  • 入库时间 2022-08-19 04:25:49
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