Simplified procedures for recovery of PHAs via physical or chemical extraction supplemented by chemical separation by difference of polarity or traditional

摘要

Simplified procedures are described for extraction and recovery of bioplastics polihidroxialcanoatos (PHAs), more specifically the chemical extraction and physical of poly - 3hidroxibutiratos [P (3HB)].Process simplification and cost reduction in these steps result in reduction of the final price of biodegradable bioplastics, stimulating their production and marketing.The present invention relates to improvement of process simplification / classic recovery by chemical extraction of PHAs with chlorinated solvents, especially in chloroform.And the development of new process for extraction and physical recovery of PHAs.The innovations of the technology proposal in relation to the classical methodology of extraction and recovery with chlorinated solvents are: (a) the abolition of the washing stage and initial cell concentrate; (b) flights were reduced.Will the heating time in the extraction step; c) replacement of the stage of filtration of the solution by liquid - liquid extraction separation, the difference of polarity.For the separation of cellular debris of extractive solution. The innovations of the technology proposal for physical extraction is the combination of preheating and adsorption on solid phase.The technologies proposed, the combinations of modifications, as well as the proposed innovations, prevents the fragmentation of the polymeric macromolecules of interest.Significantly reduce impurities and promote the yield increase in final polymer, reducing the cost of the traditional chemical extraction.The innovative technology also reduces the total volume of chlorinated solvent used.