unnatural microbial organisms with improved energy efficiency

摘要

Abstract "Unnatural Microbial Organisms with Enhanced Energy Efficiency" The invention provides unnatural microbial organisms containing enzymatic pathways and / or metabolic modifications to increase carbon flow through acetyl-coa, or oxaloacetate and acetyl-coa. Embodiments of the invention include microbial organisms having a pathway for acetylco and oxaloacetate that includes phosphocetolase (a pk pathway). organisms also have either (i) a genetic modification that increases non-phosphotransferase (non-pts) system activity for sugar uptake, and / or (ii) a genetic modification (s) to the chain of electron (cte) transport of the organism that increases the efficiency of atp production, which increases the availability of reducing equivalents or both. microbial organisms may optionally include (iii) a genetic modification that maintains, attenuates, or eliminates the activity of a phosphotransferase (pts) system for sugar uptake. The increased carbon flux through acetylcoac and oxaloacetate may be used to produce a bioderivated compound, and microbial organisms may further include a pathway capable of producing the bioderivated compound.