NON NATURAL MICROBIAL ORGANISMS WITH IMPROVED ENERGETIC EFFICIENCY

摘要

The invention provides non natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl CoA or oxaloacetate and acetyl CoA. Embodiments of the invention include microbial organisms having a pathway to acetyl CoA and oxaloacetate that includes phosphoketolase (a PK pathway). The organisms also have either (i) a genetic modification that enhances the activity of the non phosphotransferase system (non PTS) for sugar uptake and/or (ii) a genetic modification(s) to the organism s electron transport chain (ETC) that enhances efficiency of ATP production that enhances availability of reducing equivalents or both. The microbial organisms can optionally include (iii) a genetic modification that maintains attenuates or eliminates the activity of a phosphotransferase system (PTS) for sugar uptake. The enhanced carbon flux through acetyl CoA and oxaloacetate can be used for production of a bioderived compound and the microbial organisms can further include a pathway capable of producing the bioderived compound.