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USE OF TYPE II TOXIN-ANTITOXIN SYSTEM GENES TO GENOTYPE STRAINS OF MYCOBACTERIUM TUBERCULOSIS

机译:II型毒素-抗毒素系统基因在结核分枝杆菌基因型菌株中的应用

摘要

The invention relates to medicine, phthisiology and medical microbiology and can be used for identifying clinically significant strains of Mycobacterium tuberculosis, including multidrug resistant and extensively drug resistant strains and also strains with altered virulence, when working with patients. The invention comprises a novel method of genotyping strains of M.tuberculosis which is based on single nucleotide polymorphism of type II toxin-antitoxin system genes of the vapBC, higAB and mazEF superfamilies. The use of proposed pairs of oligonucleotides can be viewed as a method for diagnosing isolates of M.tuberculosis when treating tuberculosis, establishing an epidemiological picture and identifying new resistance and virulence genes. The present genotyping method is not restricted to the use of the above-mentioned genes only: combinations of other toxin-antitoxin system genes for which corresponding oligonucleotides are constructed can be used. The proposed method enables the accurate and rapid gentoyping of microbacteria in PCR mode, real-time PCR mode and multilocus PCR mode with subsequent sequencing, inter alia, in the MiSeq System ("Illumina", USA) and the GS Junior System ("Roche", Switzerland).
机译:本发明涉及医学,生理学和医学微生物学,当与患者一起工作时,可用于鉴定临床上重要的结核分枝杆菌菌株,包括多药耐药和广泛耐药菌株,以及毒力改变的菌株。本发明包括基于vapBC,higAB和mazEF超家族的II型毒素-抗毒素系统基因的单核苷酸多态性对结核分枝杆菌菌株进行基因分型的新方法。提议的寡核苷酸对的使用可以看作是一种在治疗结核病时诊断结核分枝杆菌分离株,建立流行病学图片并鉴定新的抗药性和毒力基因的方法。本基因分型方法不仅限于上述基因的使用:可以使用为其构建了相应的寡核苷酸的其他毒素-抗毒素系统基因的组合。所提出的方法使得能够在PCR模式,实时PCR模式和多基因座PCR模式下以及随后的测序中,尤其是在MiSeq系统(“ Illumina”,美国)和GS Junior系统(“ Roche”)中,对细菌进行准确,快速的细菌分型。 (瑞士)。

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