process for the extraction and purification of native type i marine colon from scales of sardine sardina pilchardus

摘要

the present invention relates to a method of acid extraction at low temperature and depurification marine collagen type 1 soluble native from the scales of sardine (sardinepilchardus).or frozen, characterized in that it comprises the steps of (1) washing with water and drying of the scales, scales, (2) (3) alkali treatment to remove non collagenous proteins indicating geneticmodification.(4) treatment with acid to remove mineral extraction, (5) u00e0froid at acid ph to solubilize the collagen fibrils from low fat, 6 nurses aggregates7) precipitation of collagen by the addition of a neutral salt (8) reconstituted collagen precipitate, and (9) by means of a bag of dialysis, dialysis.process is within a reasonable period of time to produce a collagen with a puretu00e9maximale removing almost all of the non collagen and mineral impurities.in all the collagen increasing and limiting its denaturation.in addition to the native type collagen 1avec marine receiving a substantial return.this method optimized by the response of surfacersm reduces the length of the etacide alkaline treatment, significantly reduce the duration of acid extraction.it can also help to reduce the amount of reagents used in the present case, the acidedu00e9minu00e9ralisateur acid and soda, the extractor.the method solves the problem of the management of chemicals, more sustainable and more cost-effective.the high quality collagu00e8nenatif thus obtained is suitable for food applications, encosmetfood and biomedical research.