METHOD FOR THE EXTRACTION AND PURIFICATION OF NATIVE MARINE COLLAGEN FROM SARDINA PILCHARDUS SARDINE SCALES

摘要

The present invention relates to a method for the low-temperature acid extraction and purification of soluble type I native marine collagen from fresh or frozen sardine scales (sardina pilchardus), characterised in that it comprises the steps of: 1) washing the scales with water, 2) drying the scales, 3) alkaline treatment to eliminate non-collagen proteins, 4) acid treatment to eliminate minerals, 5) cold-extraction at acid pH in order to solubilise the native collagen fibrils, 6) ultra-centrifugation to eliminate large aggregates, 7) precipitation of the collagen by adding a neutral salt, 8) re-suspension of the precipitated collagen, and 9) dialysis using a dialysis pouch. The method can be used to produce collagen having a maximum purity within a reasonable period of time, by removing almost all of the non-collagen impurities and minerals, solubilising all of the collagen and limiting the denaturation thereof. In addition to obtaining a high yield of type I native marine collagen, the method of the invention, optimised by the response surface method (RSM), can be used to reduce the duration of the alkaline and acid treatment and to considerably reduce the duration of the acid extraction. It can also be used to reduce the amount of reagents used, in this case soda, demineralisation acid and extraction acid. The method solves the particular problem of managing chemicals, in a more durable and cost-effective manner. The high-quality native collagen obtained is suitable for food, food-based cosmetics and biomedical uses.