首页> 外国专利> PRIMER SET FOR PCR FOR BACTERIAL DNA AMPLIFICATION, KIT FOR DETECTION AND/OR IDENTIFICATION OF BACTERIAL SPECIES, AND, METHOD OF DETECTION AND/OR IDENTIFICATION OF BACTERIAL SPECIES

PRIMER SET FOR PCR FOR BACTERIAL DNA AMPLIFICATION, KIT FOR DETECTION AND/OR IDENTIFICATION OF BACTERIAL SPECIES, AND, METHOD OF DETECTION AND/OR IDENTIFICATION OF BACTERIAL SPECIES

机译:用于细菌DNA扩增的PCR的试剂盒,用于细菌种类的检测和/或鉴定的试剂盒,以及用于细菌种类的检测和/或鉴定的方法

摘要

An object of the present invention is to provide a primer set useful for further improvement of sensitivity in detection or identification of bacterial species in a sample by a PCR method, a kit for PCR using the primer set and 5 a method of detection or identification of bacterial species in a sample using the primer set, as well as to further improve sensitivity in detection or identification of bacterial species in a sample by conducting a PCR method using the primer set with a minimized contamination amount of bacterial nucleic acid. A primer set according to the present invention comprises at 10 least one primer pair in seven primer pairs obtained by selecting one primer pair from each of Groups S1 to S7 consisting of specific primers in order to achieve the above objects. In addition, a kit for detection of bacterial species according to the present invention comprises at least one primer pair in seven primer pairs obtained by selecting one primer pair from each of 15 Groups K1 to K7 consisting of specific primers. 191 Fig. 1A, 1B and 1C
机译:本发明的目的是提供一种引物组,该引物组可用于进一步提高通过PCR法检测或鉴定样品中细菌种类的敏感性,一种使用该引物组的PCR试剂盒,以及5种用于检测或鉴定样品中细菌种类的方法。使用引物组的样品中的细菌物种,以及通过使用引物组进行PCR方法并最小化细菌核酸污染量的PCR方法,进一步提高检测或鉴定样品中细菌种的灵敏度。为了实现上述目的,根据本发明的引物组在七个引物对中包含至少十个引物对,所述七个引物对是通过从由特定引物组成的S1至S7组的每一个中选择一个引物对而获得的。另外,根据本发明的用于检测细菌种类的试剂盒包括在七个引物对中的至少一个引物对,所述七个引物对是通过从由特异性引物组成的15个组K1至K7的每一个中选择一个引物对而获得的。 191图1A,1B和1C

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