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PCR primer set for bacterial DNA amplification, bacterial species detection and / or identification kit, and bacterial species detection and / or identification method

机译:用于细菌DNA扩增,细菌种类检测和/或鉴定试剂盒以及细菌种类检测和/或鉴定方法的PCR引物组

摘要

An object of the present invention is to use primer sets useful for further improving sensitivity in detection or identification of bacterial species in a sample by PCR, a set for PCR using this, and detection of bacterial species in a sample or Providing an identification method, and further improving sensitivity in detection or identification of bacterial species in a sample by performing PCR using the above primer set with a minimum amount of bacterial nucleic acid contamination To achieve improvement. In order to achieve these objects, the PCR primer set for bacterial DNA amplification according to the present invention selects one primer pair for each of the groups S1 to S7 consisting of specific primer pairs. The primer pair includes at least one of the seven primer pairs obtained in the above. Furthermore, the kit for detecting a bacterial species according to the present invention is one of seven primer pairs obtained by selecting one primer pair for each of the groups K1 to K7 consisting of specific primer pairs. At least one primer pair.
机译:本发明的目的是使用引物组,该引物组用于进一步提高通过PCR检测或鉴定样品中细菌种类的灵敏度,使用该引物组的PCR以及检测样品中细菌种类的方法或提供鉴定方法,通过使用上述引物组进行PCR,并以最小量的细菌核酸污染来进一步进行PCR,从而进一步提高检测或鉴定样品中细菌种类的灵敏度,以实现改进。为了实现这些目的,根据本发明的用于细菌DNA扩增的PCR引物组为由特异性引物对组成的组S1至S7中的每一个选择一个引物对。引物对包括以上获得的七个引物对中的至少一个。此外,根据本发明的用于检测细菌种类的试剂盒是通过对由特异性引物对组成的组K1至K7的每一个选择一个引物对而获得的七个引物对之一。至少一对引物。

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