METHOD FOR EXPRESSING PROTEIN IN ASPERGILLUS NIGER HYPNOSPORES USING EXOGENOUS SINGLE STRANDED RNA

摘要

Provided is a method for expressing a protein in Aspergillus niger hypnospores by using an exogenous single-stranded RNA, comprising three steps of: culturing an Aspergillus niger and collecting spores; preprocessing Aspergillus niger pores; and electrically shocking the Aspergillus niger pores by using a HDEN method. The method uses the HDEN method to deliver in vitro single-stranded encoded protein RNA through a cell wall and a cell membrane to express the protein in Aspergillus niger hypnospores.