Method for producing negative single stranded rna virus; recombinant Plasmid functional in animal cells, consisting of a Skeleton PSS - URG; method of production of viral particles and use; to express exogenous Proteins or RNA, autologous virus.

摘要

The present invention describes a viral RNA expression plasmid and a method for obtaining viral particles based on said plasmids comprising transfecting animal cells with an expression vector or a set of expression vectors capable of expressing a nucleoprotein and RNA-dependent polymerase RNA; and transfecting the animal cell with an expression vector or a set of expression vectors with nucleotide sequences encoding recombinant RNA molecules.