LARGE CIRCULATING FLUIDIZED BED CELL BIOREACTOR AND METHOD FOR CULTURING ANIMAL CELLS

摘要

A large circulating fluidized bed cell bioreactor and a method for culturing animal cells. The reactor comprises an agitator tank body (01), an agitator tank base (50), a water inlet silicone hose (06), a backflow silicone hose (08), a cell culture tank (10), a culture tank base (40), a backflow pipe (15), a water inlet pipe (12), an agitator (20), a reactor tray (30), and a tube support plate (60). By means of cooperation among the above components, defects and shortcomings of fluidized bed cell bioreactors in the prior art including proneness to microbial contamination, a low level of dissolved oxygen, and a low working volume, are effectively resolved, advantageous in the popularization and application of this technology.