Selecting the optimal agitation conditions (rotating speed, type of impeller) is crucial in stirred tank bioreactors used for the culture of animal cells. In the present study, hydrodynamics and mixing quantities are experimentally determined by PIV and PLIF in a 20 litre bioreactor. The results shows that optimal agitation conditions in terms of homogenisation, suspension and mechanical constraints on cells are obtained if the impeller A310 (Lightnin) rotating at 49 rpm is used.
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