cell culture medium, animal cell lineage, methods for producing a conditioned culture medium, animal cells, an animal cell culture, virus in an animal cell culture, or preferably anchor-dependent diploid human, method for produce a vaccine, and virus population

摘要

"ANIMAL CELL CULTURE, ANIMAL CELL LINE, METHODS TO PRODUCE A CONDITIONED CULTURE MEDIUM, ANIMAL CELLS, ANIMAL CELL VIRUS, PREPLE, HIP, CELL, VIRUS CULTURE TO PRODUCE A VACCINE, AND, VIRUS POPULATION ". The present invention relates to a method for culturing anchor-dependent diploid, preferably human, animal cells in the absence of exogenous components of primary animal origin, and to a cell culture medium substantially free of exogenous components of primary origin. appropriate animal to perform said process. In particular the invention relates to cell culture medium comprising at least one, more preferably, various growth factors free of exogenous animal substance. The present invention also relates to a process for culturing anchor-dependent diploid, preferably human, animal cells in a medium according to the invention, involving the use of a non-animal trypsin substitute for cell passage. The invention further relates to a process for producing viruses, viral vaccines and the like.