Adaptation and Study of AIDS Viruses in Animal and Cell Culture Systems

摘要

Small animal models of HIV infection are needed to investigate variousfundamental and clinical aspects of HIV disease, i.e. host immune response, viral pathogenesis, and potential interventive therapies. Several classical and novel murine models are currently being studied for these purposes, but no one ideal model has been fully characterized. Within this report, we describe an additional murine model of HIV disease which uses sublethal gamma irradiated nude mice xenotransplanted HIV permissive CEM cells to serve as a nucleus of virus replication. Nude mice are transplanted with either 1X10/HIV chronically infected or naive CEM cells and monitored for tumor progression and total body weight gain over time.