ANTIBODY-POLYSACCHARIDE CONJUGATE, AND HIGH-SENSITIVITY IMMUNOASSAY METHOD USING SAME

摘要

The purpose of the present invention is to provide an antibody-polysaccharide conjugate for immunoassay that is obtained by binding an antibody or antibody complex to at least one side chain of a water-soluble polysaccharide via a linker, and an immunoassay method using the same.　Provided is an antibody-polysaccharide conjugate for high-sensitivity immunoassay such as a sandwich ELISA and immunochromatography, in which an antibody such as a VHH antibody or an antibody complex comprising an antibody and a cushion protein such as a stefin A mutant is bound, via a linker, to a functional group on a side chain of a water-soluble polysaccharide such as pullulan, whereby it is easier to control the orientation of the antibody when the water-soluble polysaccharide is adsorbed to a solid-phase surface for immunoassay, and it is possible for an antibody having a stable multivalent structure to bind to a target protein without the nonspecific adsorption of other proteins. Additionally provided is a high-sensitivity immunoassay method using the same.