METHOD FOR DETERMINING BISPHENOL A IN BIOLOGICAL MATERIAL, DIAGNOSTIC DEVICE FOR DETECTION OF BISPHENOL A IN BIOLOGICAL MATERIAL, DIAGNOSTIC KIT FOR DETECTION OF BISPHENOL A IN BIOLOGICAL MATERIAL

摘要

Method for determining bisphenol A in biological material that relies on binding BPA from biological material with antibodies against bisphenol A and subsequent quantitative determining of BPA content, characterized in that, a migration membrane is being prepared to determine BPA from the migrating biological material so that the membrane is divided into at least a zone of binding with a carrier, a detection zone and a control zone. Color medium covered with monoclonal or polyclonal antibodies against BPA is placed at the zone of binding with the carrier. The detection zone is divided into at least two detection areas while at the first detection area a selected concentration of antibodies against BPA is adsorbed so that BPA can be detected in the range of 0.5 ng/mL to 50 ng/mL and on the second detection area selected concentration of antibodies against BPA is adsorbed so that BPA can be detected in the 0.5 ng/mL to 50 ng/mL. On the control zone monoclonal or polyclonal antibodies against monoclonal or polyclonal antibodies against BPA. Analysed biological material is being applied at the zone of binding with carrier wherein in case of BPA presence, the BPA is being bound with color medium and thus the created complexes of BPA-color medium are directed into the detection zone in which the complexes of BPA-color medium are bound to the antibodies against BPA while the color medium not bound with BPA are directed to the control zone where the color medium is bound with the antibodies against antibodies against BPA. BPA presence is determined by color indication in at least one detection area of the detection zone and by determination of color at the control zone.