Diagnostic Kit for Simultaneously Detecting Mycobacterium Complex and Non-tuberculosis Mycobacteria

摘要

The present invention relates to diagnostic kit and method for simultaneously detecting Mycobacterium tuberculosis and non-Mycobacterium tuberculosis. According to the present invention, the kit and method have excellent sensitivity to detect up to 15 copies of Mycobacterium tuberculosis and non-Mycobacterium tuberculosis, and are compatible with gene amplification equipment. In addition, a single PCR reaction can simultaneously detect various pathogenic MTC and NTM genes. A Uracil DNA glycosylase (UDG) system is introduced to minimize cross-contamination by blocking carryover or crossover contamination by previous PCR products.