A medium compostion for lymphocyte derived from peripheral blood mononuclear cells having anticancer activity and cultivation method using the same

摘要

The present invention relates to a medium composition for culturing anticancer activated lymphocytes derived from peripheral blood mononuclear cells, and a method for culturing anticancer activated lymphocytes by using the same. In particular, the present invention has confirmed that a medium composition containing a normal hexane fraction of Melia toosendan bark, interleukin-2 (IL-2), anti-CD3 monoclonal antibodies, and anti-CD16 monoclonal antibodies has the effects of promoting differentiation of NK cells, NKT cells, and T cells (corresponding to anticancer activated lymphocytes) and mass propagation of the same from peripheral blood mononuclear cells. Accordingly, the present invention relates to a medium composition based on a normal hexane fraction of Melia toosendan bark, and a method for culturing peripheral blood mononuclear cells by using the same. In addition, the anticancer activated lymphocytes derived from peripheral blood mononuclear cells, cultured according to the method of the present invention, may be used as a pharmaceutical composition extremely effective in the treatment of cancer. Further, the medium composition of the present invention may be used in the anticancer treatment as is, and can dramatically increase anticancer activity while attenuating side effects by lowering the dose of an existing anticancer agent when administered in combination with a chemical anticancer agent in a clinical setting.